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. 2009 Aug 24;29(22):6074–6085. doi: 10.1128/MCB.00924-09

FIG. 3.

FIG. 3.

Mll1 is required for the H3K4 methylation and transcription of a subset of Hox genes. (A) Distribution of H3K4me3 and total H3 across 8-kb regions of each Hox gene promoter (5.5 kb before the transcription start site and 2.5 kb after the transcription start site) on the Agilent promoter array as determined by ChIP. Each individual Hox gene is represented as a box with an arrow indicating the major transcription start site and the direction of transcription. Vertical lines represent small alternative exons, and diagonal lines represent alternative splicing events. Reductions in H3K4me3 can be seen at several locations across the Hoxa, Hoxb, and Hoxc clusters. In contrast, Hoxd genes show little loss of H3K4me3 in Mll1−/− cells. (B) qRT-PCR analysis of the expression of Hox genes in Mll1+/+ and Mll1−/− fibroblasts normalized to the average value of reference genes Actb and Tbp. (C) Rescaling of the RT-PCR data presented in panel B. Shown are selected Hoxa and Hoxd genes, both of which have relatively low levels of expression in these fibroblasts. Some Hoxd genes that are not expressed in wild-type MEFs are expressed in the Mll1−/− MEFs. Error bars show standard deviations.