FIG. 6.
The intact MRN complex is required for Rec12-oligonucleotide formation. Meiotic inductions of a rad50Δ mutant strain (GP6730) (A and B) and an nbs1Δ mutant strain (GP6892) (C and D) are shown. Samples were treated as described for Fig. 1. Filled arrowhead, 32P-labeled Rec12-oligonucleotide complex; open arrowhead, Rec12-free protein. “+” indicates rad50+ nbs1+ extracts of strain GP6010 at 4 h. Panels B and D show DSB analysis of DNA from the meiotic inductions shown in panels A and C, respectively. Arrows, chromosome I NotI fragment J (top) and its two prominent DNA fragments broken at meiotic break sites mbs1 and mbs2.