FIG. 7.

Ctp1 is a second putative nuclease necessary for Rec12-oligonucleotide formation. Meiotic induction of ctp1⁺ control strain GP6010 (A) and ctp1Δ mutant strain GP6654 (B) is shown. Samples were treated as described for Fig. 1. Filled arrowhead, ³²P-labeled Rec12-oligonucleotide complex; open arrowhead, Rec12-free protein. (C) DSB analysis of DNA from the meiotic induction shown in panel B. Arrows, chromosome I NotI fragment J (top) and its two prominent DNA fragments broken at meiotic break sites mbs1 and mbs2.