FIG. 5.

The reduction in ush expression levels has no effect on PSC cell number or size. (A) Ush expression in the PSC was monitored by assessing the level of Ush coexpression with the PSC-specific marker Antp (confocal microscopy) (magnification, ×60). (B and C) PSC cell number was monitored using Antp in animals with altered levels of ush expression (fluorescent microscopy) (magnification, ×40). Shown are the wild type (wt) (B) and ush trans heterozygotes (C). Scale bars, 50 μm (A) and 25 μm (B).