FIG. 5.

Expression levels of TOM6 do not affect the SAM complex. (A) Mitochondria were isolated from wild-type (WT) or tom6Δ cells transformed with an empty vector (pRS426) or from wild-type cells transformed with a plasmid carrying TOM6. The given amounts of mitochondria were analyzed by sodium dodecyl sulfate (SDS)-PAGE and immunodecoration with antibodies against the indicated mitochondrial outer membrane proteins. (B) The indicated amounts of mitochondria were analyzed by BN-PAGE and immunodecoration with antibodies against Sam50. (C) Mitochondria isolated as described above were incubated with radiolabeled precursor of porin at 25°C for various time periods. Samples were then treated with proteinase K, and mitochondria were reisolated. Imported porin was analyzed by SDS-PAGE and autoradiography.