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. 2009 Sep 14;29(22):6128–6139. doi: 10.1128/MCB.00815-09

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FIG. 3. — DLC-1 promotes METT-10 nuclear accumulation. (A) METT-10::GFP expression in a wild-type germ line. (B) dlc-1 RNAi causes a decrease in METT-10::GFP accumulation, abnormal nuclear morphology (left), and unpaired chromosomes in diakinesis (right, yellow arrows). (C) METT-10::GFP nuclear accumulation is delayed in the dhc-1(or195) mutant background, with nuclear accumulation observed only in late pachytene. Solid and feather arrows indicate nuclei positive and negative for METT-10::GFP, respectively. (D) IP-Western blotting for METT-10::GFP levels in whole animals subject to both control (empty vector) RNAi and dlc-1(RNAi). CGH-1 is a germ line ubiquitous protein. (E) qRT-PCR for dlc-1 (top) and mett-10 (bottom) normalized to cgh-1 mRNA levels. RNAi was carried out in the rrf-1(pk1417) background, which retains RNAi only in the germ line.