TABLE 1.
Transgenic rescue of mett-10 phenotypese
| Transgenec | % Tumorous germ lines (no.)d |
Transgenic rescue of mett-10(ok2204) at 25°Cb |
||||
|---|---|---|---|---|---|---|
| mett-10 (ok2204) | glp-1(oz264) mett-10(g38) | Animals scored |
% Abnormal gonad shape (no.)a | |||
| % Pvl | % Ste | No. | ||||
| No transgene | 0 (96) | 89 (47) | 98 | 99 | 82 | 30 (44) |
| met-10(wt)::gfp | 0 (136) | 0 (56) | 5 | 0** | 38 | 0 (35) |
| mett-10(5A)::gfp (LOW) | 0 (86) | 0 (54) | 65 | 19** | 43 | ND |
| mett-10(5A)::gfp (HIGH) | 0 (36) | ND | 1 | 0** | 137 | 3 (32)** |
| mett-10 (enzymatic-defective, 5A)::gfp (line 1) | 0 (36) | 91 (84) | 92 | 98 | 46 | 30 (43) |
| mett-10 (enzymatic-defective, 5A)::gfp (line 2) | 0 (48) | 94 (83) | 97 | 99 | 97 | ND |
| mett-10(ΔNLS, 5A)::gfp (line 1) | 0 (37) | 4.3 (94) | 0 | 19** | 42 | 10 (31)** |
| mett-10(ΔNLS, 5A)::gfp (line 2) | ND | ND | 0 | 14** | 36 | 12 (25) |
a
Gonads with one or more ectopic distal protrusions.
b
**, two-tailed P value and Fisher's exact test value of <0.05 were significantly different from no transgene. Pvl, protruding vulva; Ste, sterile. Scored by DIC.
c
LOW and HIGH refer to low and high mRNA and protein expression levels, respectively, as measured by IP and qRT-PCR (data not shown).
d
Tumorous includes proliferation throughout the germ line, although some germ cells in various meiotic stages may be observed. Scored by DIC.
e
ND, not determined.