TABLE 2.
Dynein disruption enhances tumor formation in the background of the mett-10(oz36) cytoplasmic mutant
| Genotype | % Tumorousc | No. of tumors |
|---|---|---|
| dlc-1a | ||
| rrf-1(pk1417) mett-10(oz36) gfp(RNAi) | 3 | 117 |
| rrf-1(pk1417) dlc-1(RNAi) | 0 | 122 |
| rrf-1(pk1417) mett-10(oz36) dlc-1(RNAi) | 62 | 130 |
| Dynein heavy chainb | ||
| dhc-1(or195) | 0 | 37 |
| mett-10(oz36) | 43 | 65 |
| glp-1(oz264) | 1 | 99 |
| dhc-1(or195) glp-1(oz264) | 9 | 22 |
| dhc-1(or195) mett-10(tm2697) | 0 | 35 |
| dhc-1(or195) mett-10(g38) | 0 | 18 |
| dhc-1(or195) mett-10(oj32) | 0 | 26 |
| dhc-1(or195) mett-10(ok2204) | 0 | 65 |
| dhc-1(or195) mett-10(oz36) | 100** | 46 |
| dhc-1(or195) glp-1(bn18) mett-10(oz36) | 0 | 38 |
| dhc-1(js319) | 0 | 26 |
| dhc-1(js319) glp-1(oz264) | 6 | 17 |
| dhc-1(js319) mett-10(tm2697) | 0 | 59 |
| dhc-1(js319) mett-10(g38) | 0 | 40 |
| dhc-1(js319) mett-10(oj32) | 0 | 30 |
| dhc-1(js319) mett-10(ok2204) | 0 | 24 |
| dhc-1(js319) mett-10(oz36) | 76** | 42 |
| dhc-1(js319) glp-1(bn18) mett-10(oz36) | 0 | 52 |
a
For all genotypes, animals were m− z−, i.e., came from homozygous mutant mothers. rrf-1(pk1417) enables germ line restricted RNAi. All RNAi and scoring was carried out at 20°C.
b
For all genotypes, all animals were m+ z−, i.e., came from mothers carrying a wild-type copy of mett-10, and were scored at 2 days past L4 at 20°C by dissection and DAPI (4′,6′-diamidino-2-phenylindole) staining.
c
**, significance as calculated by Fisher's exact test, using mett-10(oz36) alone for expected values.