TABLE 1.
Overview of infected specimens tested in triplicate with our novel triplex qPCRa
| Sample group and HTLV subtype | Origin | No. of specimens | Disease manifestationb |
|---|---|---|---|
| Samples donated by IMTAvH | |||
| HTLV-1a | PBMC | 45 | AC |
| PBMC | 16 | HAM/TSP | |
| PBMC | 33 | OC | |
| Samples donated by Pasteur Institute | |||
| HTLV-1a | Human gDNA | 10 | ATLL |
| HTLV-1b | Human gDNA | 5 | ND |
| HTLV-1c | Human gDNA | 15 | ND |
| HTLV-1d | Human gDNA | 4 | ND |
| HTLV-2 | Human gDNA | 1 | ND |
| STLV-3 | Plasmid PSG5M-Tax3-pyl43 | 1 | ND |
| Samples from the Rega Institute | |||
| HTLV-1b | PBMC | 5 | ND |
| HTLV-1e | PBMC | 1 | ND |
| HTLV-1f | PBMC | 1 | ND |
| HTLV-2b | Gu cell line | 1 | ND |
| HTLV-2d | PBMC | 1 | ND |
| HTLV-2a | Cl19 cell line | 1 | ND |
| STLV-3 | PH969 cell line | 1 | ND |
| Samples donated by IMTAvH | |||
| HTLV-1a | PBMC | 8 | AC |
| PBMC | 6 | OC | |
| PBMC | 8 | HAM/TSP |
a
A total of 141 HTLV/STLV-infected specimens were used for assay performance validation of genotype and subtype detection, and 22 HTLV-1a-infected patient samples donated by IMTAvH were used for assay performance validation of PVL determination.
b
OC, other complications (uveitis, Strongyloides stercoralis infection, ID, other [scabies, genital warts]); ND, not determined.