TABLE 3.

Intra-assay and interassay reproducibility analysis of triplex HTLV and singleplex β-globin qPCR assays, based on standard templates

Analysis group, HTLV type, and dilution	μ ± σ	%CV
Intra-assay reproducibility
HTLV-1
105	22.02 ± 0.08	0.38
104	25.31 ± 0.07	0.26
103	28.84 ± 0.14	0.47
102	32.33 ± 0.17	0.53
10	36.63 ± 0.66	1.81
HTLV-2
105	21.94 ± 0.08	0.35
104	25.28 ± 0.06	0.24
103	28.70 ± 0.09	0.31
102	32.13 ± 0.19	0.58
10	35.63 ± 0.45	1.27
HTLV-3
105	22.15 ± 0.06	0.27
104	25.59 ± 0.09	0.37
103	29.12 ± 0.06	0.21
102	32.67 ± 0.26	0.80
10	35.37 ± 0.46	1.29
β-Globin
105	23.17 ± 0.05	0.23
104	26.51 ± 0.06	0.22
103	29.97 ± 0.12	0.41
102	33.46 ± 0.23	0.68
10	36.85 ± 0.80	2.18
Interassay reproducibility
HTLV-1
105	22.72 ± 0.26	1.13
104	26.94 ± 0.47	1.75
103	30.52 ± 0.52	1.70
102	34.08 ± 0.56	1.65
10	37.70 ± 1.60	4.25
HTLV-2
105	23.70 ± 0.14	0.58
104	27.30 ± 0.21	0.75
103	30.83 ± 0.22	0.70
102	34.28 ± 0.27	0.77
10	38.38 ± 1.45	3.79
HTLV-3
105	24.33 ± 0.16	0.65
104	28.11 ± 0.21	0.74
103	31.66 ± 0.31	0.98
102	35.23 ± 0.56	1.56
10	39.52 ± 2.00	5.00
β-Globin
105	21.58 ± 0.18	0.82
104	24.88 ± 0.18	0.74
103	28.37 ± 0.19	0.68
102	31.84 ± 0.25	0.77
10	35.61 ± 0.69	1.95