TABLE 4.
Intra-assay and interassay reproducibility analysis of triplex HTLV and singleplex β-globin qPCR assays, based on four infected patient samples
| Analysis group and patient sample no. | HTLV-1 copies |
β-Globin copies |
PVL |
|||
|---|---|---|---|---|---|---|
| Mean ± SD | %CV | Mean ± SD | %CV | Mean ± SD | %CV | |
| Intra-assay reproducibility | ||||||
| 1 | 12.0 ± 1.0 | 8.2 | 6,829.4 ± 416.1 | 6.1 | 24.7 ± 1.9 | 7.5 |
| 2 | 21.1 ± 3.5 | 16.5 | 7,888.0 ± 188.6 | 2.4 | 37.5 ± 6.5 | 17.4 |
| 3 | 1.4 ± 0.2 | 14.5 | 13,204.1 ± 503.2 | 3.8 | 1.5 ± 0.2 | 15.6 |
| 4 | 57.2 ± 8.6 | 15.0 | 6,229.7 ± 47.4 | 0.8 | 128.6 ± 18.4 | 14.3 |
| Interassay reproducibility | ||||||
| 1 | 9.1 ± 2.7 | 29.4 | 6,595.0 ± 585.3 | 8.9 | 19.3 ± 5.3 | 27.6 |
| 2 | 15.8 ± 5.0 | 31.4 | 7,029.5 ± 1,591.6 | 22.6 | 31.5 ± 7.5 | 23.7 |
| 3 | 1.8 ± 0.4 | 24.4 | 13,518.8 ± 454.8 | 3.4 | 1.9 ± 0.4 | 22.6 |
| 4 | 61.6 ± 7.9 | 12.7 | 6,142.3 ± 531.0 | 8.6 | 140.7 ± 15.5 | 11.0 |