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. 2009 Sep 9;47(11):3682–3691. doi: 10.1128/JCM.00781-09

TABLE 4.

Intra-assay and interassay reproducibility analysis of triplex HTLV and singleplex β-globin qPCR assays, based on four infected patient samples

Analysis group and patient sample no. HTLV-1 copies
β-Globin copies
PVL
Mean ± SD %CV Mean ± SD %CV Mean ± SD %CV
Intra-assay reproducibility
    1 12.0 ± 1.0 8.2 6,829.4 ± 416.1 6.1 24.7 ± 1.9 7.5
    2 21.1 ± 3.5 16.5 7,888.0 ± 188.6 2.4 37.5 ± 6.5 17.4
    3 1.4 ± 0.2 14.5 13,204.1 ± 503.2 3.8 1.5 ± 0.2 15.6
    4 57.2 ± 8.6 15.0 6,229.7 ± 47.4 0.8 128.6 ± 18.4 14.3
Interassay reproducibility
    1 9.1 ± 2.7 29.4 6,595.0 ± 585.3 8.9 19.3 ± 5.3 27.6
    2 15.8 ± 5.0 31.4 7,029.5 ± 1,591.6 22.6 31.5 ± 7.5 23.7
    3 1.8 ± 0.4 24.4 13,518.8 ± 454.8 3.4 1.9 ± 0.4 22.6
    4 61.6 ± 7.9 12.7 6,142.3 ± 531.0 8.6 140.7 ± 15.5 11.0