TABLE 1.
Results from serology (ELISA), PCR,a and isolation of CvHV2 in cell culture for unaffected animals and for each group of animals with clinical symptoms of IKCb
| Group (no. of animals) | No. of ELISA-positive animals/total no. tested (%) | No. of PCR-positive swab samples/total no. tested |
Virus isolation |
|||
|---|---|---|---|---|---|---|
| Eye | Nasal | Vaginalc | No. of eye swabs for which CvHV2 was isolated | Sum of CPE scoresd | ||
| Unaffected animals (grade 0) (12) | 5/12 (42) | 5/12 | 2/12 | 3/11 | 0 | 0 |
| Animals with clinical symptoms | ||||||
| Grade 1 (6) | 5/6 | 5/6 | 3/6 | 1/4 | 1 | 3 |
| Grade 2 (5) | 4/5 | 4/5 | 2/5 | 1/5 | 3 | 9 |
| Grade 3 (7) | 5/7 | 6/7 | 5/7 | 1/6 | 3 | 11 |
| Grade 4 (3) | 3/3 | 3/3 | 2/3 | 0/3 | 1 | 3 |
| Grade 5 (5) | 5/5 | 3/5 | 5/5 | 3/5 | 0 | 0 |
| NAe (2) | 2/2 | 2/2 | 1/2 | 0/2 | 0 | 0 |
| Total (28) | 24/28 (86) | 23/28 | 18/28 | 6/25 | 8/28 | 26 |
| All animals (40) | 29/40 (73) | 28/40 | 20/40 | 9/36 | 8/40 | 26 |
a
Of the UL27 gene, encoding gB.
b
Animals with clinical symptoms are grouped according to the severity of the symptoms, from mild (grade 1) to severe (grade 5).
c
For vaginal swabs, the total number of animals tested is the number of the females in each group.
d
Calculated by adding the individual CPE scores for all animals from whose eye swabs CvHV2 was isolated. Individual CPE scores range from 1 (slightly cytopathic) to 4 (highly cytopathic).
e
NA, not assessed.