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Primers used for construction of RSP5 derivatives for expression in E. coli and yeast
| Primer pair | RSP5 derivative (amino acid position) |
|---|---|
| 2796/1849 | Full-length Rsp5 (1-809) |
| 2796/2806 | C2 (1-206) |
| 2796/2800 | C2/WW (1-443) |
| 2799/1849 | HECT (445-809) |
| 2805/1849 | WW/HECT (208-809) |
| 2805/2800 | WW1-3 (208-443) |
| 2805/2802 | WW1-2 (208-383) |
| 2803/2800 | WW2-3 (308-443) |
| 2796/2804 | C2/WW1 (1-306) |
| 2801/1849 | WW3/HECT (385-809) |
