FIG. 4.

Overexpression of PRMT6 increases the half-life of Tat. (A) HeLa cells expressing Tat-FLAG with or without Myc-PRMT6 were treated with CHX and harvested at 0, 2, 4, and 6 h posttreatment. Western blotting was performed on the total-protein-equalized lysates. The β-tubulin levels show equivalent sample loadings. (B) The Tat-FLAG band intensities in panel A were quantified, and their natural log values were plotted as a function of time. Values for Tat-FLAG with Myc-PRMT6 are indicated by black boxes and values for Tat-FLAG alone by white boxes. The calculated Tat-FLAG half-lives are shown. The data points represent the means and standard deviations of two independent experiments.