FIG. 6.

Silencing REGγ expression does not affect the ability of PRMT6 to stabilize Tat. HeLa-derived cell lines stably expressing miRNA against PSME3 (REGγ miR +) or a nontargeting negative control miRNA (REGγ miR −) were transfected to express Tat-FLAG with or without Myc-PRMT6. All samples were cotransfected with β-galactosidase vector. The cells were treated with CHX (CHX +) or vehicle (CHX −) for 3 h before Western blotting was performed on β-galactosidase-equalized lysates (for Myc-PRMT6 and Tat-FLAG blots) or total-protein-equalized lysates (for REGγ and β-tubulin blots). The Tat-FLAG bands were quantified and are expressed relative to lane 3, where columns represent mean band intensities and error bars indicate standard deviations. The data are representative of three independent experiments.