FIG. 4.

Analysis of HDAC1 and HDAC2 in cells infected with adenoviruses expressing wild-type and kinase-deficient ORF66p. MeWo cells were mock infected or infected with AdEmpty, AdORF66WT, or AdORF66KD at a multiplicity of infection of 10 in conjunction with AdTetOff at a multiplicity of infection of 5. As a positive control, MeWo cells were infected with cell-associated wild-type VZV at a multiplicity of infection of 0.025. At 48 hpi, cells were harvested and equal amounts of total protein (20 μg) were analyzed by Western blotting using antisera specific for HDAC1, HDAC2, GFP, and HSP90.