FIG. 2.
Long-term gene expression in memory cells is not due to viral latency. B6 mice were infected with 2 × 105 PFU of LCMV Armstrong. (A) Intracellular granzyme B expression was analyzed on days 8 and 60 postinfection for the indicated epitope-restricted populations (black line). The isotype control staining of the same cell populations also is shown (dashed line). The profile shown is representative of results obtained from three individual mice. (B) An in vivo cytotoxicity assay also was performed on days 8 and 60 postinfection, and specific cytotoxicity is depicted; the results shown are the mean of data from three individual mice, and the error bars indicate standard errors of the means.