FIG. 6.

BiP associates with pp28 and TRS1 during infection. (A) Lysates from mock (M)- and HCMV (V)-infected HFs were prepared at 96 hpi for immunoprecipitation with BiPY, an antibody that detects BiP in the assembly compartment, and the KDEL antibody, which does not detect BiP in the assembly compartment. Following immunoprecipitation, reactions were analyzed by Western blotting using antibodies against BiP, TRS1, and pp28. Western analysis of 20% of the input is also shown (Input). (B and C) Similar lysates from mock- and HCMV-infected cells were immunoprecipitated with anti-pp28, and Western blots were probed with anti-BiP (B) or anti-TRS1 (C). A cross-reaction with light chains by the pp28 antibody is shown (LC) in panels B and C.