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. 2009 Sep 2;83(22):11491–11501. doi: 10.1128/JVI.01214-09

FIG. 2.

FIG. 2.

CryoEM structure of the HAdV12/αvβ5 integrin complex. (A) Full structure viewed along a twofold icosahedral axis and shown as three radial shells: icosahedral capsid (300 to 463 Å, blue), integrin ring and fiber (443 to 515 Å, gold), and diffuse integrin density (515 to 600 Å, red). The icosahedral capsid shell is shown filtered to 8 Å with an applied B-factor of −300 Å2, the integrin ring and fiber filtered to 19 Å with an applied B-factor of −500 Å2, and the diffuse integrin density filtered to 33 Å with an applied B-factor of −500 Å2. (B) Enlarged top and side views of the vertex region colored as in panel A with the fiber shaft in green. The diffuse integrin density (red) is shown with a lower isosurface contour level in panel B (1.2σ versus 1.7σ in panel A) to display a fuller extent of this density. Scale bars, 100 Å. (C) An FSC plot indicating a resolution range for the HAdV12/αvβ5 icosahedral capsid (radial shell, 300 to 463 Å) of 8.3 to 6.9Å (8.3 Å, FSC = 0.5; 7.5 Å, FSC = 0.3; 6.9 Å, FSC = 0.143) (blue); the HAdV12/αvβ5 radial shell (443-515Å) including the integrin ring and fiber above the penton base of 27Å to 19Å (27 Å, FSC = 0.5; 24 Å, FSC = 0.3; 19 Å, FSC = 0.143) (gold); and the HAdV12/αvβ5 radial shell (515-600Å) with diffuse integrin density of 85Å to 33Å (85 Å, FSC = 0.5; 50 Å, FSC = 0.3; 33 Å, FSC = 0.143) (red).