FIG. 8.

Comparison of HAdV12/αvβ5 integrin density with bent and extended integrin models. (A) The cryoEM integrin density (mesh) is shown with four copies of the integrin ectodomain in a bent conformation. This is a composite integrin model built from PDB-IDs 1L5G (43) and 18UC (42). (B) The penton base is shown with four copies of the integrin ectodomain in an extended conformation. This is a composite integrin model built from the domains shown in Fig. 5 with the remaining domains modeled to approximate the cryoEM density in the outermost radial shell (515 to 600 Å) of the HAdV12/αvβ5 structure. In both panels the αv chains are blue, the β chains are red, the bound RGD peptides are green, and the penton base is gold. Note that the gap between the upper and lower cryoEM integrin density regions is an artifact from calculating the density in separate radial shells.