TABLE 1.

Transposition rates and cDNA production

Strain group	Strain	MAT type	Descriptiona	Transposition at 24°Cb	cDNAc
Wild type	SWY2283	a	wt	+++	1.0
	SWY2284	α	wt	+++	1.0
Single FxF(G) and GLFG deletion mutants	SWY2801	α	nup1ΔFxFG	+++	ND
	SWY2731	a	nup2ΔFxFG	+++	0.4
	SWY2772	a	nup60ΔFxF	+++	ND
	SWY2922	a	nsp1ΔFGΔFxFG	+++	1.0
	SWY2825	a	nup49ΔGLFG	++	0.8
	SWY2869	a	nup145ΔGLFG	++	0.4
	SWY2754	a	nup57ΔGLFG	++++	0.6
	SWY2764	a	nup100ΔGLFG	++++	0.5
	SWY2789	a	nup116ΔGLFG	+++	4.2/1.7**
Double GLFG deletion mutants	SWY2882	a	nup49ΔGLFG nup57ΔGLFG	++	1.2
	SWY2916	a	nup116ΔGLFG nup145ΔGLFG	+	1.6/2.5**
	SWY2819	a	nup116ΔGLFG nup57ΔGLFG	+++*	1.7/2.2**
	SWY2841	a	nup116ΔGLFG nup49ΔGLFG	+	1.7
	SWY2973	a	nup100ΔGLFG nup145ΔGLFG	++	0.5
	SWY2785	a	nup100ΔGLFG nup57ΔGLFG	+++	0.5
	SWY2835	a	nup100ΔGLFG nup49ΔGLFG	++	ND
	SWY2924	a	nup145ΔGLFG nup57ΔGLFG	++	0.7
Triple symmetric deletion mutants	SWY2968	a	nup100ΔGLFG nup145ΔGLFG nup49ΔGLFG	−	5.3***
	SWY2951	a	nup100ΔGLFG nup145ΔGLFG nup57ΔGLFG	+	1.3
	SWY2953	α	nup100ΔGLFG nup145ΔGLFG nup57ΔGLFG	+	1.3
	SWY3012	a	nup100ΔGLFG nup57ΔGLFG nsp1ΔFGΔFxFG	+	1.5
	SWY2980	a	nup100ΔGLFG nup145ΔGLFG nsp1ΔFGΔFxFG	++	1.8
Asymmetric mutants	SWY2844	a	ΔC	+++	0.5
	SWY2852	α	ΔC nsp1ΔFG	++++	1.8***
	SWY2897	α	ΔN	++	0.4
	SWY2905	α	ΔN nsp1ΔFxFG	+++	0.7/1.2**
	SWY3062	a	ΔNΔC nsp1ΔFGΔFxFG	++	1.26
	SWY3063	α	ΔNΔC nsp1ΔFGΔFxFG	+	0.9***
	SWY2971	α	ΔNΔC	++	0.2
Asymmetric + GLFG deletions	SWY3462	a	ΔNΔC nup145ΔGLFG	++	0.3
	SWY3410	α	ΔNΔC nup57ΔGLFG	+++	0.7
	SWY3930	a	ΔNΔC nup57ΔGLFG	+++	1.4
	SWY3043	a	ΔNΔC nup100ΔGLFG	+++	0.3
Slow-growing strains	SWY2965	a	nup145ΔGLFG nup49ΔGLFG	ND	0.5
	SWY2871	a	nup100ΔGLFG nup49ΔGLFG nup57ΔGLFG	ND	1.2
	SWY3008	a	nup100ΔGLFG nup49ΔGLFG nsp1ΔFGΔFxFG	ND	No cDNA
	SWY3603	α	ΔNΔC nup116ΔGLFG	ND	No cDNA

^aΔC, Nup42ΔFG Nup159ΔFG; ΔN, Nup1ΔFxFG Nup2ΔFxFG Nup60ΔFxF.

^bCells that had undergone transposition formed papillations on selective medium, and the phenotype was scored for three to four transformants for each mutant (+ to ++++; − indicates no papillations). *, results shown are for three of four transformants tested (reduced for one of four).

^cFor cDNA analysis, two transformants were tested unless stated otherwise. The cDNA-to-plasmid ratio was quantified using Quantity One software (Bio-Rad, Hercules, CA) and was normalized to the ratio for the wt, which was designated as 1. One of the two transformants is shown if the difference between levels of cDNA compared to wt was less than 0.2. **, results shown are for two transformants with a cDNA difference of more than 0.2; ***, only one transformant was tested; ND, not determined.