FIG. 2.

HIV-2 RodA Env expression mediates cell surface tetherin downregulation in HeLa cells. HeLa (A) or 293T/THN-HA (B) cells were transfected with a control empty vector (EV) or vectors encoding the indicated viral proteins linked to enhanced GFP via an IRES. At 48 h posttransfection the cells were stained for cell surface tetherin levels with a monoclonal anti-BST-2 antibody (A) or a monoclonal anti-HA (B) antibody and then a secondary goat-anti-mouse Alexa 633 antibody. The cells were then analyzed by flow cytometry. Median fluorescence intensities are indicated for the boxed regions.