TABLE 1.
Primers and probes designed for the one-step multiplex real-time RT-PCR
| Primer or probe | Sequence (5′-3′) | Nucleotide positionsa |
|---|---|---|
| MGBGI_F | ATGTTCCGCTGGATGC | 5285-5300 |
| MGBGI_R | CCTTAGACGCCATCWTCAT | 5360-5376 |
| MGBGI_Probe | VIC-TGGACAGGRGATC-MGB | 5321-5333 |
| MGBGII_F | ATGTTYAGRTGGATGAGRTT | 5012-5031 |
| MGBGII_R | ACGCCATCTTCATTCACA | 5080-5097 |
| MGBGII_Probe | FAM-TGGGAGGGCGATC-MGB | 5048-5060 |
a
GI primer and probe nucleotide positions are based on those of Hu/NoV/GI.1/Norwalk virus/1968/US; GII primer and probe nucleotide positions are based on those of Hu/NoV/GII.4/MD145-12/1987/US.