TABLE 1.
Summary of Southern blot resultsa
| Cell line | Vector | MOI | Vector ratio | No. of clones with AAV sequences | % Integrationb |
|
|---|---|---|---|---|---|---|
| AAVS1 | Random | |||||
| M059J | P5UF11:SVAV2 | 106 | 50:1 | 25 | 72 | 28 |
| dsP5AAVNeoR:dsSVRep78 | 104 | 5:1 | 21 | 23.8 | 76.2 | |
| M059K | P5UF11:SVAV2 | 106 | 50:1 | 25 | 52 | 48 |
| dsP5AAVNeoR:dsSVRep78 | 104 | 5:1 | 17 | 23.5 | 76.5 | |
| Ligase IV | P5PGKHygro:SVAV2 | 105 | 50:1 | 25 | 20 | 80 |
| HeLa | P5UF11:SVAV2 | 105 | 50:1 | 11 | 45 | 54 |
| dsP5AAVNeoR:dsSVRep78 | 105 | 50:1 | 8 | 0 | 100 | |
a
Genomic DNA from neomycin- or hygromycin-resistant clones was analyzed with Southern blotting by hybridizing to AAVS1 and neomycin/hygromycin-specific probes.
b
Colinking of AAVS1 and neomycin/hygromycin-specific signals was used to determine the percent AAVS1 and random integration. The percent AAVS1 and random integration was determined using the number of clones with detectable vector sequences.