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Coimmunoprecipitation of EBNA1 with nucleosome assembly proteins. Nucleosome assembly proteins were immunoprecipitated from Raji nuclear lysates by use of antibodies against NAP1, NAP2, TAF-I (α and β forms), or TAF-Iβ, and recovered proteins were Western blotted (WB) for EBNA1, NAP1, NAP2, or TAF-I. Immunoprecipitation was also performed with a nonspecific antibody (IgG) as a negative control. A 1/20 sample of the nuclear lysate used for immunoprecipitation is also shown (input).
