FIG. 2.

Glycerol gradient sedimentation analysis of EBNA1-nucleosome assembly protein complexes. Equal molar ratios of EBNA1 and NAP1 (A) or EBNA1 and TAF-Iβ or TAF-Iα (B) were preincubated, analyzed on a glycerol gradient, and compared to the same proteins analyzed individually. Equal-volume fractions were collected from the top of each gradient and analyzed by SDS-PAGE and silver staining, and the pellet at the bottom of the tube (P) is also shown. Sedimentation positions are shown (at the top) for the molecular mass markers aldolase (158 kDa) and catalase (232 kDa), which were analyzed on an identical gradient. A sample of the protein(s) loaded on the gradients is also shown (input).