FIG. 3.

Effects of nucleosome assembly proteins on EBNA1 solubility. (A) EBNA1 and NAP1 were incubated alone or together (at an equal molar ratio) in buffer containing 50 to 250 mM NaCl. Soluble (S) and precipitated (P) proteins were then separated by centrifugation and analyzed by SDS-PAGE and Coomassie blue staining. A sample of the protein(s) prior to incubation is shown as “input.” The same experiment was also performed with an EBNA1 mutant lacking amino acids 325 to 376 (EBNA1Δ325-376). (B) Same experiment as in panel A, except that TAF-Iβ, TAF-Iα, or NAP2 was used in place of NAP1.