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. 2009 Sep 9;83(22):11926–11939. doi: 10.1128/JVI.01008-09

FIG. 3.

FIG. 3.

RNA binding of JFH1 and J6 NS5B measured by an SPR biosensor. (A) Interaction between immobilized NS5B variants (JFH1, black; J6, gray) and template RNA [3.3 μg/ml 3′(+) JFH1 RNA; 0.86 μg/ml 3′(+) J6 RNA], corresponding to the 3′ region of the respective positive-strand genome. (B) Interaction between immobilized NS5B variants (JFH1, black; J6, gray) and the 3′ portion of the JFH1 or J6 negative-strand RNA [50 μg/ml 3′(−) JFH1 RNA; 3.1 μg/ml 3′(−) J6 RNA], each 341 nt in length [3′(−)341nt] (Fig. 5A). The SPR biosensor response scale on the y axis was normalized so that 1 corresponds to a 1:1 stoichiometry (RNA-NS5B).