TABLE 1.

Mamu-B*08-restricted CD8⁺ T-cell epitopes mutated in 8X-SIVmac239^a

Epitope	Amino acid sequenceb	Mamu-B*08 binding affinity (IC50 [nM])c	Fold reduction in binding to Mamu-B*08
Vif172-179RL8	RRDNRRGL	125
	.G......	594	4.8d
Vif123-131RL9	RRAIRGEQL	7.5
	.K.......	2,901	387d
Nef137-146RL10	(A)RRHRILDIYL	11
	(P)..........	NT	NAe
Nef246-254RL9b	RRLTARGLL	3.2
	........P	477	151d
Env573-581KL9	KRQQELLRL	12
	........M	397	33d
Rev12-20KL9	KRLRLIHLL	3.2
	.....L...	1.6	Nonef
Nef8-16RL9a	RRSRPSGDL	105
	....Q....	428	4.1g
Rev44-51RL8	RRRWQQLL	20
	......I.	59	2.9f

^aEpitopes are listed in order of the typical immunodominance hierarchy of responses targeting the epitopes. Subscript numbers in the epitope name indicate amino acid positions of the epitope.

^bThe mutations introduced are indicated beneath the sequence of the epitope. These changes are indicated in boldface type.

^cPeptides with IC₅₀s of >500 nM are not considered binders and are indicated in italics. The binding of WT and 8X variant peptides to Mamu-B*08 was described previously (33). NT, not tested.

^dThe putative mechanism of escape is a primary anchor residue mutation.

^eThe putative mechanism of escape is a processing mutation. NA, not applicable, as the mutation introduced is outside the defined epitope.

^fThe putative mechanism of escape is a TCR contact mutation.

^gThe putative mechanism of escape is TCR contact mutation along with some reduction in Mamu-B*08 binding.