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Accumulation of SeV proteins during coinfection with HRV1a. HeLa cells were infected with HRV1a for 8 h (lane 1); with SeV for 3, 5, 7, or 15 h (lanes 3 to 5); or with HRV1a for 5 h followed by SeV for 3 or 5 h (lanes 7 and 8). No sample was loaded in lane 6. Cell extracts were prepared and fractionated by SDS-PAGE, and SeV proteins were detected by Western blot analysis using anti-SeV antiserum. α, anti.
