FIG. 3.

Hypoxia induces NOR-1 expression in HUVEC. NOR-1 (A) and VEGF (B) mRNA levels from HUVEC cultured under normoxia (Norm) or exposed to hypoxia (1% O₂), CoCl₂ (1 mM), or deferoxamine mesylate (DFO; 100 μM) for 4 h. (C and D) Hypoxia induces NOR-1 expression in a dose- and time-dependent manner. (C) HUVEC were cultured under normoxia (Norm) or exposed to hypoxia or increasing concentrations of CoCl₂ for 4 h. (D) Time-dependence experiments performed in cells subjected to hypoxia (1% O₂) or CoCl₂ (1 mM) for increasing times. (E and F) VEGF mRNA levels from cells cultured as indicated for panels C and D. Results were normalized by 18S rRNA. Data are from three independent experiments performed in triplicate. *, P < 0.05 versus controls.