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. 2009 Aug 31;29(21):5828–5842. doi: 10.1128/MCB.00945-09

FIG. 6.

FIG. 6.

CREB is not involved in hypoxia-induced NOR-1 upregulation. (A) Representative Western blot showing the effect of hypoxia (1% O2) or CoCl2 (1 mM) on CREB activation for increasing times. The activation of CREB by VEGF (50 ng/ml) is shown as a positive control. Norm, normoxia. (B) Representative Western blot showing the reduction of CREB protein levels in cells transfected with an siRNA against CREB (siCREB) and cultured under normoxia (Norm) or exposed to hypoxia (1% O2) or CoCl2 (1 mM) for 4 h. An siRNA against a control silencer sequence (siControl) was used. Unchanged levels of β-actin are shown as a loading control. (C and D) mRNA levels of NOR-1 (C) and VEGF (D) from cells transfected with siCREB (+) and exposed to hypoxia for 4 h. Data are from three independent experiments performed in triplicate. siCREB (−) indicates cells transfected with siControl. *, P < 0.05 versus cells transfected with siControl and exposed to normoxia.