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mRNAs for cell cycle proteins are elevated when the Rb1 pathway is mutated in TKO MEFs, but Arf mRNA is not induced, nor is it induced in response to activated Ras. (A) Real-time PCR comparing mRNA levels in wild-type and TKO MEFs. (B) Real-time PCR for Arf mRNA compared in wild-type (WT), TKO, and TKO Ras MEFs. The error bars indicate standard deviations.
