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Mutation of the Rb1 pathway provides resistance to anoikis, shRNA knockdown of mTor restores anoikis, and mTor overexpression in wild-type (Wt) MEFs confers resistance to anoikis. Cells were trypsinized from tissue culture plates and placed in suspension culture for 18 h. Trypan blue exclusion was used to assess cell viability. The error bars indicate standard deviations.
