FIG. 7.

Knockdown of mTor using lentiviral shRNA does not significantly alter cell morphology or proliferation. (A) Low-power phase-contrast image of TKO MEFs infected with mTor shRNA lentivirus. (B) GFP immunofluorescence in panel A, showing infected cells. (C) Merge of panels A and B. (D) Higher-power phase image of TKO MEFs infected with mTor shRNA lentivirus. (E) GFP immunofluorescence in panel D. (F) Merge of panels D and E. (G) Sorting for mTor shRNA lentivirus-infected TKO MEFs based on GFP expression. (H) Analysis of cell proliferation in the indicated cells. The error bars indicate standard deviations.