FIG. 4.

Increased ROS production in myeloid cells lacking Bcr and Abr. (A) SDS lysates of BMM and neutrophils from wild-type and null mutant mice were analyzed using Abr antiserum (10) or anti-Bcr N20 antibodies by Western blotting (top and bottom, respectively). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a loading control. (B and C) Elevated superoxide production in PEM from null mutant mice. Thioglycolate-elicited macrophages from wild-type and single-null mutant mice (B) or wild-type and double-null mutants (C) were stimulated with 150 nM PMA in the presence of cytochrome c. Results were calculated as nanomoles of reduced cytochrome c/milligram of protein/time and then converted to differences (n-fold) for comparison. Assays were performed in triplicate. For panel B, four wild-type mice and five abr^−/− and bcr^−/− mice from two independent, representative experiments were used. For panel C, 3 mice each for both wild-type and abr × bcr^−/− mice from one representative experiment are shown (*, P = 4 × 10⁻⁹). Error bars indicate the standard deviations for each mean.