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. 2009 Aug 24;29(21):5751–5762. doi: 10.1128/MCB.00415-09

FIG. 5.

FIG. 5.

Phosphorylation of HDAC4 S246 is required for robust interaction of HDAC4 with 14-3-3 proteins and retention of HDAC4 in the cytoplasm. (A) USCs were transfected with an expression vehicle encoding HDAC4-WT-Flag, HDAC4-S246A-Flag, or HDAC4-3SA-Flag and cultured in either the absence or presence of PTHrP or forskolin as indicated. The percentage of chondrocytes displaying nuclearly localized HDAC4-Flag when cultured in either the absence or presence of PTHrP or forskolin is displayed. (B and C) USCs were transfected with a GAL4-firefly luciferase reporter and an SV40-renilla luciferase reporter plus expression vehicles encoding 14-3-3-VP16; MEF2-VP16; and Gal4-HDAC4(2-740)WT, Gal4-HDAC4(2-740)S246A, or Gal4-HDAC4(2-740)3SA as indicated. Relative luciferase units (RLU) are displayed. X, either a phosphate group or hydrogen.