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HOXD13 expression does not increase p53 or γ-H2AX levels. The top shows an immunoblot analysis of the expression of p53 in HEK293 cells transduced with LD13IΔN or LXIΔN. The bottom panel shows immunofluorescence staining of LXIΔN- or LD13IΔN-transduced HEK293 cells using the indicated antibodies. As a control, cells were treated with adriamycin to induce DNA damage and to promote the accumulation of p53- and γ-H2AX-positive foci.
