FIG. 9.

HOXD11 and HOXA13 bind human DNA replication origins. ChIP of endogenous HOXD11 and HOXA13 binding to human origins in HEK293 cells. Graphs represent the average enrichment of HOXD11- or HOXA13-bound origin regions (ori), as assessed by semiquantitative PCR. Values indicate the ratio (in percent) between the amount of DNA in immunoprecipitates (IP) and that in input chromatin (INPUT). Error bars represent the mean percent IP/INPUT ratio ± S.E.M. of at least three independent ChIPs for each sample. Two genomic regions, located respectively 5′ (5′c) and 3′ (3′c) to each origin were chosen as controls (see the text).