FIG. 2.

ALIX and TSG101 depletion does not affect growth of HSV-1. HaCaT (A and B) or HeLa (C) cells were transfected with specific siRNA in order to deplete the endogenous ALIX and TSG101 proteins. The siRNA- and mock-treated cells were subsequently infected with HSV-1 strain SC16 at 5 PFU/cell. Infected cells were scraped and sonicated at various time points (A and B) or 16 h postinfection (C) to release infectious progeny virus. Infectious HSV-1 titers were determined for each sample by plaque assay on Vero cells. The data represent mean PFU per ml, and error bars indicate the standard errors of the mean of triplicate samples. Protein samples from each siRNA-treated and untreated (mock) condition were analyzed by Western blotting to demonstrate the expression levels of endogenous TSG101 and ALIX at the time of infection for panels A and C or at 25 h postinfection for panel B (right-hand panels).