FIG. 2.

Viral conversion is delayed and reduced in CHO cells expressing the β-propeller-negative LDLR compared to wt LDLR. Radiolabeled HRV2 was attached to cells grown in six-well plates at 4°C for 1 h, unbound virus was washed away, and the cells were incubated in 1 ml of infection medium at 34°C, allowing for virus internalization and uncoating. At the time points indicated, the cells were lysed without removing the incubation medium. Converted virus was immunoprecipitated with MAb 2G2 and remaining native virus was immunoprecipitated with anti-HRV2 antiserum and scintillation counted. The percentage of virus conversion was calculated as 2G2-precipitated counts divided by total precipitated counts and set to 0% at time zero. Note the delay and lower extent of uncoating when the virus enters via the truncated receptor. Error bars indicate the means ± the standard deviations (SD) (n = 3).