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Growth properties of TBEV-2A in BHK-21 (A) and IRE/CTVM18 (B) cells. The cells were infected at a low MOI (0.01 for BHK-21 cells and 0.1 for IRE/CTVM18 cells). Samples were taken at different time points, and the infectivity titers were determined using a focus assay. All data points represent geometric mean values from two independent experiments. The error bars indicate the standard deviations. Inocula that were not completely removed by the washing steps are indicated by asterisks.
