FIG. 1.
Infection with MRV induces SG formation at early times p.i. HeLa (first row), MEF (second row), CV-1 (third row), and DU-145 (fourth row) cells were infected with MRV T3DC virions (1,000 PFU/cell). At 4 h p.i., cells were fixed and immunostained with rabbit anti-MRV core polyclonal antiserum (left column) and mouse monoclonal antibody against G3BP (first row, right column), goat anti-TIA-1 (second row, right column), or goat anti-TIAR (third and fourth rows, right column) polyclonal antibodies, followed by Alexa 594-conjugated donkey anti-rabbit IgG and Alexa 488-conjugated donkey anti-mouse IgG or donkey anti-goat IgG. More than 200 infected cells were counted on each slide, and the percentage of infected cells containing SG-like granules at the time of fixation is indicated. Scale bars = 10 μm.