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. 2009 Aug 26;83(21):11090–11101. doi: 10.1128/JVI.01239-09

FIG. 3.

FIG. 3.

MRV-induced SGs contain translation initiation factors eIF3, eIF4G, and eIF4E but not P body component DCP1a. HeLa cells were infected with T1L ISVPs (1,000 PFU/cell) for 2 h and then fixed and immunostained with mouse anti-λ2 monoclonal antibody 7F4 (Core) (left column); goat anti-TIA-1 or goat anti-TIAR antibodies (second column); and rabbit anti-eIF3, eIF4G, and eIF4E antibodies or DCP1a antisera (third column); followed by Alexa 594-conjugated donkey anti-mouse IgG, Alexa 488-conjugated donkey anti-goat IgG, and Alexa 350-conjugated donkey anti-rabbit IgG. Merged images are shown in the right column. Scale bars = 10 μm.