FIG. 10.

CypA expression level in HeLa and Jurkat cells. (A) Cell lysates from HeLa and Jurkat cells were normalized by total protein concentrations and subjected to Western blotting analysis. The blots were probed with an anti-CypA antibody and an HRP-conjugated anti-GAPDH antibody. The results from two independent experiments (Exp 1 and Exp. 2) are shown. (B) The ratio between CypA protein levels in HeLa cells and Jurkat cells, after normalizing to the corresponding GAPDH levels, was calculated based on quantification of protein band densities. The results from two independent experiments (Exp 1 and Exp. 2) are shown.