FIG. 3.

Thin-section electron microscopy of HIV-1 virions. Virions were purified from the supernatant of 293T cells transfected with plasmids encoding the wild-type or mutant HIV-1 Gag/Pol proteins and fixed with glutaraldehyde for thin-section electron microscopy analysis. (A) Examples of virions with evident conical cores are shown. (B) The indicated number (n) of virion particles was examined for each of the HIV-1 variants. The percentage of virions in which classical conical cores were evident was calculated for each variant. wt, wild type.