FIG. 2.
Anti-HIV-1 activity of CM from uNK cells is increased by cytokine activation. (A) CM from uNK and bNK clones collected after 3 days of stimulation with IL-12 and IL-15 (S) or in the absence of cytokine stimulation (U) were preincubated for 1 h with TZM-bl cells prior to the addition of HIV-1IIIB. The percentage of inhibition of HIV-1 infection was determined by comparing RLU values with those of the medium control. Each data point represents an individual clone, and the average of each group is shown. Stimulated uNK clones showed significantly higher inhibition than unstimulated uNK clones (**, P < 0.01; n = 31), and CM from bNK clones did not inhibit HIV-1IIIB (n = 10). These data represent uNK clones from 15 individuals and bNK clones from 6 individuals. (B) Comparison of anti-HIV activities between CM from paired IL-12- plus IL-15-stimulated and unstimulated uNK clones. These data show that stimulation of uNK cells resulted in an increase in HIV-1 inhibitory activity. ***, P < 0.001.