FIG. 4.

uNK cells that inhibited HIV-1 infection produced CXCL12 and not IFN-γ. (A) Cell-free uNK CM from clones that demonstrated anti-HIV- activity (+) or lacking anti-HIV-1 activity (−) were analyzed for IFN-γ. There was a negative correlation between anti-HIV-1 activity and amounts of IFN-γ. ***, P < 0.001 (n > 6 uNK clones). (B) CM from uNK and bNK clones were analyzed for the presence of CXCL12. There was a positive correlation between CXCL12 production and anti-HIV-1 activity. ***, P < 0.001 (n = 8). Data are shown as means ± SEM. (C) Comparison between amounts of CXCL12 and IFN-γ in uNK CM. These data show that uNK CM with large amounts of CXCL12 had small amounts of IFN-γ and vice versa. Statistical analysis was performed using the Mann-Whitney U test.