FIG. 5.
Neutralizing CXCL12 abrogates uNK cell anti-HIV activity. (A) uNK clones were stimulated with IL-12 plus IL-15 for 3 days, and uNK CM (black bars) or culture medium (open bars) was preincubated with or without CXCL12 neutralizing antibodies or the isotype control (IgG). This mixture was then added to TZM-bl cells for 1 h before infecting the cells with HIV-1IIIB. β-Gal expression was determined after 48 h. (B) uNK CM from four different individuals were treated in four different experiments as in panel A, and the data were normalized to media (M). Values represent means ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (n = 4). The y axis shows RLU values, converted into percentages with “media” as 100%. Statistical analysis was performed using the Mann-Whitney U test.