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(A) SDS-PAGE analysis of purified eE2 in the presence and absence of β-mercaptoethanol (β-ME) and stained with Coomassie blue. (B) Purified eE2 was applied to a Superdex 200 size exclusion column equilibrated with 50 mM HEPES (pH 7.5), 150 mM KCl, 5% glycerol. The arrows denote the positions of the void volume, dimer, and monomer.
